Weed Science - University of Wisconsin

A System to Control Perennial Broadleaf Weeds in Glyphosate Resistant Soybeans

Jerry Doll

Roundup (glyphosate) has been a useful tool for perennial weed management for many years. It has been used in soybean production systems as either a fall or spring treatment before planting, as a spot treatment or via selective applicators in the growing crop, and as a preharvest treatment. Safety to the soy-beans was due to application timing (before or after harvest or after crop maturity) or with selective equipment (rope wick and wiper applicators). The recent development and commercialization of glyphosate-tolerant crops allow us to apply glyphosate to the growing crop and maintain selectively in a manner similar to that of other selective herbicides: the crop is able to physiologically protect itself from the normally lethal effect by which glyphosate kills weeds.

This development is revolutionizing our ability to manage several perennial weeds, especially broadleaf species with spreading root systems like hemp dogbane, common milkweed, Canada thistle, perennial sow-thistle, Jerusalem artichoke and field and hedge bindweed. These weeds are persistent in all tillage systems. The availability of glyphosate resistant crops opens the door for us to apply the optimum rate of a highly systemic herbicide at the ideal growth stage of the perennial weed with essentially no risk of crop injury and we still have crop competition to further weaken the weed infestation.

Most of the soybeans grown in Wisconsin are planted in narrow rows to maximize yield potential. This also enhances the competitive advantage of soybeans with weeds by shading the soil surface sooner than when the crop is planted in 20-inch or wider rows. Also, many of our soybeans are planted without tillage. This is a distinct advantage to maximize the effectiveness of glyphosate on perennial weeds for two reasons. First, in no-till systems weed growth is uninterrupted and the weeds reach more advanced growth stages sooner than if tillage were performed. This means that perennial reach the bud to early flowering stage earlier than if tillage were done, and because this is the time when translocation to the root is maximized, the best long term control should result in a no-till system. Also the absence of tillage helps ensure that the root system is intact and that most of the below ground system will have the chance to receive glyphosate from treated shoots.

1995 to 1998 Field Research

We have researched perennial weed management in glyphosate resistant soybeans since 1995 to find the most effective time and rate of glyphosate application to control three of our common perennial broadleaf weeds. Two trials each were done on hemp dogbane, Canada thistle and common milkweed and the results have led us to propose a program that manages annual weeds, 2) gives long term suppression of the perennial species, and 3) does not increase the number of trips over the field.

All trials were done in grower fields in Wisconsin, were planted by the producer, and were done in narrow row, no-till (or near no-till) production systems. Either a burn down herbicide application or a light disking was done to kill emerged annual weeds before planting. Roundup was applied in 15 to 18 gal/acre of water when the weed was in the late vegetative to bud stage or in the early flowering growth stage. Rates of glyphosate applied were .56, .75, 1.125, and 1.5 lb ai/acre (these rates correspond to 1.5, 2.0, 3.0, and 4.0 pints/acre of Roundup or Roundup Ultra) in single applications and combinations of the lower rates as repeated applications (the second application usually 7 to 14 days after the first). A summary of the weed and soybean growth stages and planting and treatment times is found in Table 1.

Control ratings were taken several times during the season. Weed reinfestation ratings and were taken at the end of the growing season and again in June of the following year. Weed populations the year after applications were also determined.

Summary

Hemp dogbane was most sensitive to Roundup when in the early and full flowering stages. Sequential applications of glyphosate that started with hemp dogbane in the early bud stage gave control similar to that of single applications made to plants in the early and full flowering stages. The 1.5 and 2.0-pint/acre rates of Roundup applied in the early to full flowering growth stages approached eradication after a single use in glyphosate resistant soybeans.

Roundup gave excellent control of Canada thistle at most rates and times of application, including the higher Roundup rated applied at the early application time. The 1.5 and 2.0-pint Roundup rates applied to thistles in the early flowering stage also appeared to eradicate thistles in just a single season. Canada thistle is more sensitive to glyphosate than hemp dogbane.

Treating common milkweed in the late vegetative to early bud stage (30 days after planting) was less effective than treating in the early flowering stage. Roundup at 3.0 or 4.0 pints/acre applied to milkweed in the early flowering stage and in split treatments gave the best kill of treated plants. However, some milkweed plants escaped control at each date because they emerged after the application. Split applications were no better than a single application in the early flowering stage.

Overall control of the treated perennial broadleaf weeds in glyphosate resistant soybeans was excellent and several treatment timings and rates approached eradication. Non-uniform weed emergence was especially noticeable with hemp dogbane and common milkweed. Canada thistle emerged both more uniformly and earlier than the other species studied.

Thus, the right time to treat perennial broadleaves is at the late bud to early flowering stage. This is often 5 to 7 weeks after planting when the soybeans are in the V-4 growth stage (Table 1) and is later than when annual weeds would normally be treated. However, this later timing is consistently the best time for perennials because at this growth stage herbicide movement from the treated foliage to the roots is maximized.

We never decided to apply Roundup based on the calendar. The number of days from planting until we made the early (usually bud stage) and late (usually early flowering stage) applications show why (Table 1). The days to reach these growth stages varied considerably across years, sites and weeds. On average, the early applications were made 32 days after planting and the later ones at 42 days. Soybeans at these times were approaching the two trifoliate leaf stage for the early applications and averaged 4 trifoliate leaves for the later timing.

Putting the system together: annual and perennial weed
control in glyphosate resistant soybeans

Based on these observations and other considerations, we have arrived at the following recommendations for perennial broadleaf weed management in Roundup Ready soybeans. In addition, a no-till (or near no-till) system does not disturb the weed roots before treatment.

 Plant the crop without tillage. Tillage delays the development of perennial weeds while in a no-till system, the weed grows rapidly and reaches the ideal growth stage for treatment sooner than if tillage were done.

 Apply a reduced rate (perhaps 50%) of the recommended rate of a soil-active herbicide with the burn down treatment before planting to avoid crop yield loss due to uncontrolled annual weeds while waiting to treat perennial broadleaves. Select the preemergence herbicide(s) based on the expected annual weed population and product price. A reduced rate is not risky because any escaping annual weeds will be killed by the glyphosate application targeted to the perennial weed. In fields with perennial broadleaf weeds where tillage has been done, apply a reduced rate of a PPI or PRE herbicide. It may take longer for the perennial species to reach the flowering stage in these fields.

 Delay the glyphosate application until the first flowers appear on the perennial broadleaf weed or until the weed is 24 to 30 inches tall, whichever occurs first. Perennial broadleaf weeds do not develop uniformly. Not all plants will flower or be at the same height at the same time. Apply these guidelines to the most advanced plants in the population. Our applications in the "early flowering" stage meant that at least some plants were flowering but most were not.

 Application timing is more important than glyphosate rate. Our research has shown that applying Roundup at 2.0 to 3.0 pints/acre to actively growing perennial weeds in the early flower stage gives excellent control the season of application with greatly reduced weed populations the next year. This illustrates the tremendous effect of soybean competition on perennial broadleaves by not allowing the weed to replenish the root reserves before going dormant in the fall.

 Split applications of glyphosate are not necessary. Control from a single treatment when perennial broadleaves begin to flower is as good as that of repeated treatments. This means that no additional trips through fields with perennial broadleaf weeds should be needed as a well-timed single postemergence application (following the burn down treatment with a residual product) will give maximum effect on the perennial weeds.

 Uniform coverage of the weed foliage is important and difficult. As mentioned, weed height is seldom uniform in a population of perennial broadleaves. Select the appropriate nozzles and adjust the boom height to cover the weed foliage as uniformly as possible. Remember that boom height also affects the risk of particle drift from the target area.

 Monitor the perennial weed population in following years and use an appropriate management program if/when they again reach threshold levels.

The Future

Our research to date has focused on single season use of glyphosate in glyphosate resistant crops. We now need information on the best systems to handle perennial weeds for the long term. Systems to test in comparison to single season use of glyphosate are:

 alternate year use of a glyphosate resistant crop

 consecutive year use of glyphosate resistant crops (2, 3 or 4 seasons) and

 alternate control strategies in rotation crops, for example clopyralid (Stinger), for Canada thistle control in corn in rotation with glyphosate in glyphosate resistant crops.

Research on the interaction of tillage with on perennial weed management in glyphosate resistant crops is also needed.

Table 1. Planting dates, days after planting, and soybean growth stage when perennial broadleaf weeds were treated with glyphosate in the vegetative to bud and early flowering growth stages in trials for the trials in 1995 to 1998.

Year and Weed

Planting Date Vegetative bud stage - days after planting Early flowering stage - days after planting Vegetative bud stage - crop growth stage Early flowering stage - crop growth stage
95 Dogbane May 16 31 37 1 trifoliate 2-3 trifoliates
96 Dogbane May 17 34 42 1-2 trifoliate 3 trifoliates
97 Canada thistle May 22 25 36 1 trifoliate 4 trifoliate
97 Milkweed May 13 30 39 1-2 trifoliates 3-4 trifoliates
98 Canada thistle May 9 44 55 3-4 trifoliates 6-7 trifoliates
98 Milkweed May 20 28 43 1-2 trifoliates 4-5 trifoliates
Average . 32 42 1.7 trifoliates 4 trifoliates

February 1999

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